Coding

Part:BBa_K2980013:Design

Designed by: Zikang Huang   Group: iGEM19_Tsinghua   (2019-10-16)


lacI-GFP-FUS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1377
    Illegal NheI site found at 1400
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

lacI should be added at N terminal of the fused protein. Linker between lacI and GFP is needed.


Source

Part:BBa K2980005 Part:BBa K2980008 Part:BBa_K1088019

References